Antibody neutralization of a myotoxin from the venom of Bothrops asper (terciopelo)
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| Authors: | , , , |
|---|---|
| Format: | artículo original |
| Publication Date: | 1987 |
| Description: | Neutralization of biological activities of B. asper myotoxin by a monospecific anti-myotoxin rabbit serum and polyvalent antivenom was studied. Both antisera neutralized myotoxic, phospholipase A and lethal activities of myotoxin, whereas edema-forming activity of myotoxin was not neutralized by these antisera. Anti-myotoxin rabbit serum neutralized most of the myotoxic activity of B. asper venom, but did not neutralize phospholipase A activity of this venom. Neutralization curves showed that myotoxicity induced by myotoxin persisted at antivenom/toxin or antiserum/toxin ratios at which phospholipase A activity was completely neutralized. This suggests that myotoxicity does not depend upon enzymatic activity of the toxin. Antiserum to myotoxin neutralized approximately 70% of myonecrosis induced by crude B. asper venom. This demonstrates that myotoxin is the main factor responsible for the development of myonecrosis in envenomations by B. asper. |
| Country: | Kérwá |
| Institution: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/29148 |
| Online Access: | http://www.sciencedirect.com/science/article/pii/004101018790078X https://hdl.handle.net/10669/29148 |
| Keyword: | Animals Antivenins Crotalid Venoms Muscles Necrosis Neutralization Tests Phospholipases A Snake venom |