Neutralization of myotoxic phospholipases A2 from the venom of the snake Bothrops asper by monoclonal antibodies
Wedi'i Gadw mewn:
| Awduron: | , , , |
|---|---|
| Fformat: | artículo original |
| Dyddiad Cyhoeddi: | 1992 |
| Disgrifiad: | The neutralization of two myotoxic phospholipases A2 from the venom of Bothrops asper, myotoxins I and II, by two murine monoclonal antibodies is reported. The monoclonal antibodies, MAb-3 and Mab-4, recognize different epitopes of the toxin. Both antibodies completely neutralized myotoxic activity of myotoxin I and crude venom. MAb-3 also completely neutralized myotoxic activity of myotoxin II, a lysine-49 phospholipase A2 isoform, whereas MAb-4 neutralized this toxin only partially. MAb-3 neutralized myotoxin II at a molar ratio of 1:1, showing a higher efficiency than affinity-purified polyclonal antibodies. A dissociation of myotoxic and enzymatic activities of myotoxin I was observed with both monoclonal antibodies. |
| Gwlad: | Kérwá |
| Sefydliad: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/29173 |
| Mynediad Ar-lein: | https://hdl.handle.net/10669/29173 |
| Allweddair: | Animals Antibodies Monoclonal Crotalid Venoms Epitopes Group II Phospholipases A2 Mice Inbred BALB C Neurotoxins Neutralization Tests Phospholipases A Phospholipases A2 Reptilian Proteins |