Discovery and optimization of a broadly-neutralizing human antibody against long-chain α-neurotoxins from snakes

 

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Bibliografski detalji
Autori: Ledsgaard, Line, Wade, Jack, Jenkins, Timothy P., Boddum, Kim, Oganesyan, Irina, Harrison, Julian A., Villar, Pedro, Leah, Rachael A., Zenobi, Renato, Schoffelen, Sanne, Voldborg, Bjørn, Ljungars, Anne, McCafferty, John, Lomonte, Bruno, Gutiérrez, José María, Laustsen, Andreas Hougaard, Karatt Vellatt, Aneesh
Format: artículo original
Datum izdanja:2023
Opis:Snakebite envenoming continues to claim many lives across the globe, necessitating the development of improved therapies. To this end, broadly-neutralizing human monoclonal antibodies may possess advantages over current plasma-derived antivenoms by offering superior safety and high neutralization capacity. Here, we report the establishment of a pipeline based on phage display technology for the discovery and optimization of high affinity broadly-neutralizing human monoclonal antibodies. This approach yielded a recombinant human antibody with superior broadly-neutralizing capacities in vitro and in vivo against different long-chain α-neurotoxins from elapid snakes. This antibody prevents lethality induced by Naja kaouthia whole venom at an unprecedented low molar ratio of one antibody per toxin and prolongs the survival of mice injected with Dendroaspis polylepis or Ophiophagus hannah whole venoms.
Zemlja:Kérwá
Institucija:Universidad de Costa Rica
Repositorio:Kérwá
Jezik:Inglés
OAI Identifier:oai:kerwa.ucr.ac.cr:10669/89111
Online pristup:https://www.nature.com/articles/s41467-023-36393-4
https://hdl.handle.net/10669/89111
Access Level:acceso abierto
Ključna riječ:human antibody
monoclonal antibody
snake venom
neurotoxin
neutralization
VENENOS ANIMALES

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