Discovery and optimization of a broadly-neutralizing human antibody against long-chain α-neurotoxins from snakes

 

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Détails bibliographiques
Auteurs: Ledsgaard, Line, Wade, Jack, Jenkins, Timothy Patrick, Boddum, Kim, Oganesyan, Irina, Harrison, Julian A., Villar, Pedro, Leah, Rachael A., Zenobi, Renato, Schoffelen, Sanne, Voldborg, Bjørn, Ljungars, Anne, McCafferty, John, Lomonte, Bruno, Gutiérrez, José María, Laustsen, Andreas Hougaard, Karatt Vellatt, Aneesh
Format: artículo original
Date de publication:2023
Description:Snakebite envenoming continues to claim many lives across the globe, necessitating the development of improved therapies. To this end, broadly-neutralizing human monoclonal antibodies may possess advantages over current plasma-derived antivenoms by offering superior safety and high neutralization capacity. Here, we report the establishment of a pipeline based on phage display technology for the discovery and optimization of high affinity broadly-neutralizing human monoclonal antibodies. This approach yielded a recombinant human antibody with superior broadly-neutralizing capacities in vitro and in vivo against different long-chain α-neurotoxins from elapid snakes. This antibody prevents lethality induced by Naja kaouthia whole venom at an unprecedented low molar ratio of one antibody per toxin and prolongs the survival of mice injected with Dendroaspis polylepis or Ophiophagus hannah whole venoms.
Pays:Kérwá
Institution:Universidad de Costa Rica
Repositorio:Kérwá
Langue:Inglés
OAI Identifier:oai:kerwa.ucr.ac.cr:10669/89111
Accès en ligne:https://www.nature.com/articles/s41467-023-36393-4
https://hdl.handle.net/10669/89111
https://doi.org/10.1038/s41467-023-36393-4
Mots-clés:human antibody
monoclonal antibody
snake venom
neurotoxin
neutralization
VENENOS ANIMALES

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