Preclinical evaluation of the efficacy of antivenoms for snakebite envenoming: State-of-the-art and challenges ahead

 

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Bibliographic Details
Authors: Solano Trejos, María Gabriela, Pla Ferrer, Davinia, Herrera Vega, María, Segura Ruiz, Álvaro, Vargas Arroyo, Mariángela, Villalta Arrieta, Mauren, Sánchez Brenes, Andrés, Sanz, Libia, Lomonte, Bruno, León Montero, Guillermo, Calvete Chornet, Juan José, Gutiérrez, José María
Format: artículo original
Publication Date:2017
Description:Animal-derived antivenoms constitute the mainstay in the therapy of snakebite envenoming. The efficacy of antivenoms to neutralize toxicity of medically-relevant snake venoms has to be demonstrated through meticulous preclinical testing before their introduction into the clinical setting. The gold standard in the preclinical assessment and quality control of antivenoms is the neutralization of venom-induced lethality. In addition, depending on the pathophysiological profile of snake venoms, the neutralization of other toxic activities has to be evaluated, such as hemorrhagic, myotoxic, edema-forming, dermonecrotic, in vitro coagulant, and defibrinogenating effects. There is a need to develop laboratory assays to evaluate neutralization of other relevant venom activities. The concept of the 3Rs (Replacement, Reduction, and Refinement) in Toxinology is of utmost importance, and some advances have been performed in their implementation. A significant leap forward in the study of the immunological reactivity of antivenoms against venoms has been the development of “antivenomics”, which brings the analytical power of mass spectrometry to the evaluation of antivenoms. International partnerships are required to assess the preclinical efficacy of antivenoms against snake venoms in different regions of the world in order to have a detailed knowledge on the neutralizing profile of these immunotherapeutics
Country:Kérwá
Institution:Universidad de Costa Rica
Repositorio:Kérwá
OAI Identifier:oai:kerwa.ucr.ac.cr:10669/74727
Online Access:http://www.mdpi.com/2072-6651/9/5/163/htm
https://hdl.handle.net/10669/74727
Access Level:acceso abierto
Keyword:Antivenoms
Neutralization tests
Preclinical efficacy
Antivenomics
The 3Rs
Snake venom