Butanol production by Clostridium beijerinckii BA101 using cassava flour as fermentation substrate: enzymatic versus chemical pretreatments
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| Autores: | , , , , |
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| Formato: | artículo original |
| Fecha de Publicación: | 2011 |
| Descripción: | Cassava flour (CF), a cost-effective source of starch, was employed as a substrate for successful acetone butanol-ethanol (ABE) production by batch-fermentation with Clostridium beijerinckii. The effect of temperature, initial concentration of CF and chemical/enzymatic hydro lysis were studied in a 23 factorial design. Results revealed that temperature and initial concentration of substrate exert a significant effect on ABE production, as well as interac tions of temperature with the other variables. Solvent pro duction was maximized when working at 40 C, 60 g l-1 CF and enzymatic pretreatment. An average of 31.38 g l-1 ABE was produced after 96 h, with a productivity of 0.33 g l-1 h-1 . A posterior randomized block design (3 9 2) showed that enzymatic hydrolysis (with sacchari fication periods of 6 h at 60 C) enhances both reducing sugar and solvent production if compared to chemical pretreatments. Average ABE production in this case was 27.28 g l-1 , with a productivity of 0.28 g l-1 h-1 . Results suggest that CF may be a suitable substrate for industrial ABE production. |
| País: | Kérwá |
| Institución: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| Lenguaje: | Inglés |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/90815 |
| Acceso en línea: | https://hdl.handle.net/10669/90815 |
| Palabra clave: | CASSAVA Butanol ABE fermentation Hydrolysis Clostridium beijerinckii |