Extraction and amplification of DNA from orchid exsiccates conserved for more than half a century in a herbarium in Bogotá, Colombia

 

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Библиографические подробности
Авторы: Mazo, Laura C., Gómez, Alberto, Quintanilla, Sonia R., Bernal, Jaime E., Ortiz Valdivieso, Pedro
Формат: artículo original
Статус:Versión publicada
Дата публикации:2013
Описание:Plant tissue from herbarium specimens contains DNA that has undergone post-mortem degradation. Only small amounts of possibly degraded genetic material free of chemicals and impurities can be extracted from these samples. The aim of the present work was to compare and determine which one of three previously published plant DNA extraction protocols would extract good quality DNA from orchid herbarium specimens stored up to 63 years, susceptible of PCR amplification and Sanger sequencing. The most effective protocol for this type of samples allowed us to obtain and sequence amplified products in 6 of the 7 samples studied with an optimal DNA / protein purity relationship.
Страна:Portal de Revistas UCR
Институт:Universidad de Costa Rica
Repositorio:Portal de Revistas UCR
Язык:Inglés
OAI Identifier:oai:portal.ucr.ac.cr:article/11762
Online-ссылка:https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/11762
Ключевое слово:dna
adna
orchids
matk
exsiccates
herbarium