Extraction and amplification of DNA from orchid exsiccates conserved for more than half a century in a herbarium in Bogotá, Colombia
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| Авторы: | , , , , |
|---|---|
| Формат: | artículo original |
| Статус: | Versión publicada |
| Дата публикации: | 2013 |
| Описание: | Plant tissue from herbarium specimens contains DNA that has undergone post-mortem degradation. Only small amounts of possibly degraded genetic material free of chemicals and impurities can be extracted from these samples. The aim of the present work was to compare and determine which one of three previously published plant DNA extraction protocols would extract good quality DNA from orchid herbarium specimens stored up to 63 years, susceptible of PCR amplification and Sanger sequencing. The most effective protocol for this type of samples allowed us to obtain and sequence amplified products in 6 of the 7 samples studied with an optimal DNA / protein purity relationship. |
| Страна: | Portal de Revistas UCR |
| Институт: | Universidad de Costa Rica |
| Repositorio: | Portal de Revistas UCR |
| Язык: | Inglés |
| OAI Identifier: | oai:portal.ucr.ac.cr:article/11762 |
| Online-ссылка: | https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/11762 |
| Ключевое слово: | dna adna orchids matk exsiccates herbarium |