Brucella intergenic-OME: Genomic characterization of intergenic regions unveiling structural similarities and differences within chromosomes and Brucella species
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| Autorzy: | , , , |
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| Format: | texto |
| Status: | Versión publicada |
| Data wydania: | 2026 |
| Opis: | Brucella species exhibit a high level of genomic similarity, with whole-genome compar- isons revealing 97-99% identity among species. Despite this genetic homogeneity, distinct differences in host preference, virulence and zoonotic potential contribute to the unique infection outcome of each species. Intergenic regions, which comprise approximately 15% of prokaryotic genomes, are non-coding DNA sequences located between genes and play critical roles in regulating gene expression, impacting different cellular processes such as environmental adaptation and mobilization of genetic elements. In this study, we generated separated intergenic content profiles (Intergenic-OME) for chromosomes I and II, for twenty Brucella strains, to determine structural similarities and highlight differences within intergenic regions across Brucella species. Our findings reveal structural variation in intergenic content between chromosomes I and II among all analyzed Brucella species. Intergenic regions accounted for 12-14% of chromosome I and 10-12% of chromosome II con- tent. Notably, chromosome I harbors longer intergenic regions, and shorter coding regions than chromosome II. The GC content of intergenic regions range from 49-51%, confirming that these are relatively AT-rich compared to overall Brucella genomic GC content. No significant differences were found in GC content between chromosomes or in the orien- tation patterns of genes (unidirectional, convergent or divergent) either within or across species. Phylogenetic analyses were performed for each chromosome based on intergenic- OME data; to determine how intergenic regions structural similarities and differences by chromosome support and contribute to dilucidate relations among Brucella species. These preliminary results underscore the relevance of intergenic regions in comparative genomics and highlight the need for complementary in vivo and functional analysis to fully elucidate their biological significance. |
| Kraj: | Portal de Revistas UNA |
| Instytucja: | Universidad Nacional de Costa Rica |
| Repositorio: | Portal de Revistas UNA |
| Język: | Inglés |
| OAI Identifier: | oai:www.revistas.una.ac.cr:article/22474 |
| Dostęp online: | https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/22474 |
| Słowo kluczowe: | Brucella |