Micropropagation of Superior Clones of Mahogany (Swietenia macrophylla King) from Nodal Segments
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| Autores: | , |
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| Formato: | artículo original |
| Estado: | Versión publicada |
| Fecha de Publicación: | 2019 |
| Descripción: | Mahogany is the most economically important native forest species in Latin America. It is listed in Appendix II of CITES because it has a high exploitation rate due to decades of high extraction rates. The objective of this research was to develop a protocol for the in vitro establishment of two clones (33 and 80), that belong to a forest genetic improvement program. Nodal segments of clones 83 and 33 were established under in vitro conditions using 15 % calcium hypochlorite with an exposure time of 20 minutes. The best culture medium for in vitro establishment of the nodes was MS supplemented with 3% sucrose, 0.016 g/L of agrimycin and benlate, whose use resulted in establishment in 55% of nodes after 16 days of culture. In both clones, the effect of five concentrations of 6-Benzylaminopurine (6-BAP) (0.0, 0.44, 0.88, 1.33, 1.77, and 2.21 μM L-1) on sprouting was evaluated. On average, one sprout per explant was obtained in the 5 treatments evaluated. No significant statistical differences were observed. |
| País: | Portal de Revistas UNA |
| Institución: | Universidad Nacional de Costa Rica |
| Repositorio: | Portal de Revistas UNA |
| Lenguaje: | Español |
| OAI Identifier: | oai:ojs.www.una.ac.cr:article/12080 |
| Acceso en línea: | https://www.revistas.una.ac.cr/index.php/ambientales/article/view/12080 |
| Palabra clave: | Benzylaminopurine; forestry species; genetic improvement program; in vitro cultivation; nodal segments. bencilaminopurina; cultivoin vitro; especie forestal; nudos; programa mejoramiento genético. benzilaminopurina; cultivo in vitro; espécie florestal; nós; programa de melhoramento genético. |