First report of a Multidrug-Resistant ST58 Escherichia coli Harboring Extended-Spectrum Beta-Lactamase of the CTX-M-1 Class in a Fecal Sample of a Captive Baird’s Tapir (Tapirus bairdii) in Costa Rica, Central America
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| Autores: | , , , , , |
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| Formato: | artículo |
| Fecha de Publicación: | 2022 |
| Descripción: | Objective: This study’s main objective was to analyze the antibiotic susceptibility profile of Escherichia coli isolates obtained from a fecal sample of a captive Baird’s tapir (Tapirus bairdii) in Costa Rica. Materials and Methods: The fecal sample was collected inside the enclosure on March 3, 2017, right after the animal defecated. Samples were cultured on MacConkey agar plates nonsupplemented and supplemented with 2 mg/mL of cefotaxime. Bacterial identification and antibiotic susceptibility were performed with the Vitek 2 Compact System and the Kirby Bauer disk diffusion method, respectively. Polymerase chain reaction ampli- fication was performed to detect blaCTX-M beta-lactamase genes. Resistant isolates were subjected to whole- genome sequencing (WGS). Results: After evaluating several antibiotic classes, a multidrug-resistant E. coli strain with extended-spectrum beta-lactamase phenotype was isolated. Resistance to cefotaxime, cefepime, ampicillin, ampicillin/sulbactam, and tetracycline was detected. WGS analysis showed the presence of blaCTX-M-1, blaTEM-1B, and tet(B) genes. The presence of IncN plasmids and Col156 was also detected. Conclusion: Our findings are according with the notion that animals’ high density enhances the spread of resistant determinants in a captive environment in a limited space, where the likelihood of direct or indirect contact with other animals and humans is more frequent. |
| País: | Repositorio UNA |
| Institución: | Universidad Nacional de Costa Rica |
| Repositorio: | Repositorio UNA |
| Lenguaje: | Inglés |
| OAI Identifier: | oai:null:11056/24359 |
| Acceso en línea: | https://www.researchgate.net/publication/353498321 http://hdl.handle.net/11056/24359 |
| Palabra clave: | COSTA RICA ESCHERICHIA COLI TAPIRUS BAIRDII DANTA ÁREAS DE PROTECCIÓN CAPTIVITY ANTIBIÓTICOS ANTIBIOTIC RESISTANCE |