Feasibility of individual carotenoid quantification in mixtures using UV-Vis spectrophotometry with multivariate curve resolution alternating least squares (MCR-ALS)
Guardado en:
| Autores: | , , , |
|---|---|
| 格式: | artículo original |
| Fecha de Publicación: | 2022 |
| 实物特征: | A fast and low-cost analytical method to determine the concentrations of carotenoids (β-carotene, lutein, and lycopene) from mixed standard solutions or from fruit extracts (kiwi fruits, tomato paste, pink grapefruit juice, kiwi-pineapple smoothie, and apricot nectar) was tested. The methodology was based on UV-Vis spectrophotometry and Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS). Results showed that relative concentrations of β-carotene, lutein, and lycopene in solvent were successfully determined by this technique with an error inferior to 6%. In real extracts, the procedure succeeded well in identifying the major carotenoid type of the fruit samples but also a more complex profile of a fruit mixture. The results also showed that accuracy of carotenoids determination by UV-Vis spectrophotometry-MCR-ALS in fruit extracts was conditioned by their spectral characteristics (III/II ratios and λmax), their relative proportion, and the extract purity |
| País: | Kérwá |
| 机构: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| 语言: | Inglés |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/89251 |
| 在线阅读: | https://www.hindawi.com/journals/jspec/2022/4509523/ https://hdl.handle.net/10669/89251 https://doi.org/10.1155/2022/4509523 |
| Palabra clave: | Carotenoids Fruits Spectrophotometry High performance liquid chromatography Quantification |