Antioxidant and Anti-inflammatory in Vitro Activities of Phenolic Compounds from Tropical Highland Blackberry (Rubus adenotrichos)
Gorde:
| Egileak: | , , , , , , , |
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| Formatua: | artículo original |
| Argitaratze data: | 2013 |
| Deskribapena: | This study evaluates the antioxidant and anti-inflammatory activities in a polyphenol extract from blackberries. The antioxidant activity measured via oxygen radical absorbance capacity (ORAC) was higher for the blackberry extract (4339 ± 144 μM TE/g) than for quercetin and ellagic acid. The blackberry phenolic compounds protected liposomes and liver homogenates against lipid peroxidation; in both models, the antioxidant activity (IC50 = 7.0 ± 0.5 and 20.3 ± 4.2 μg/mL, respectively) was greater than that found with Trolox. The extract inhibited superoxide production by NADPH oxidase in THP-1 cells and nitrite production in J774A.1 cells stimulated with LPS+IFNγ, with nitrite production decreasing after 4 h of incubation with the extract, mainly through a strong scavenging activity. However, 24 h of treatment reduced the amount of nitrites (IC50 = 45.6 ± 1.2 μg/mL) because of a down-regulation of iNOS protein expression, as demonstrated by Western blotting. The inhibitory activities found in blackberry phenols suggest a potential beneficial effect against oxidative stress and inflammatory processes. |
| Herria: | Kérwá |
| Erakundea: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| Hizkuntza: | Inglés |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/87732 |
| Sarrera elektronikoa: | https://pubs.acs.org/action/doSearch?AllField=Antioxidant+and+Anti-inflammatory+in+Vitro+Activities+of+Phenolic+Compounds https://hdl.handle.net/10669/87732 |
| Gako-hitza: | Blackberry Rubus adenotrichos Antioxidant activity Lipid peroxidation Anion superoxide iNos Macrophages cell lines AGRICULTURA FITOQUÍMICA |