Discovery and optimization of a broadly-neutralizing human antibody against long-chain α-neurotoxins from snakes

 

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Autores: Ledsgaard, Line, Wade, Jack, Jenkins, Timothy P., Boddum, Kim, Oganesyan, Irina, Harrison, Julian A., Villar, Pedro, Leah, Rachael A., Zenobi, Renato, Schoffelen, Sanne, Voldborg, Bjørn, Ljungars, Anne, McCafferty, John, Lomonte, Bruno, Gutiérrez, José María, Laustsen, Andreas Hougaard, Karatt Vellatt, Aneesh
格式: artículo original
Fecha de Publicación:2023
实物特征:Snakebite envenoming continues to claim many lives across the globe, necessitating the development of improved therapies. To this end, broadly-neutralizing human monoclonal antibodies may possess advantages over current plasma-derived antivenoms by offering superior safety and high neutralization capacity. Here, we report the establishment of a pipeline based on phage display technology for the discovery and optimization of high affinity broadly-neutralizing human monoclonal antibodies. This approach yielded a recombinant human antibody with superior broadly-neutralizing capacities in vitro and in vivo against different long-chain α-neurotoxins from elapid snakes. This antibody prevents lethality induced by Naja kaouthia whole venom at an unprecedented low molar ratio of one antibody per toxin and prolongs the survival of mice injected with Dendroaspis polylepis or Ophiophagus hannah whole venoms.
País:Kérwá
机构:Universidad de Costa Rica
Repositorio:Kérwá
语言:Inglés
OAI Identifier:oai:kerwa.ucr.ac.cr:10669/89111
在线阅读:https://www.nature.com/articles/s41467-023-36393-4
https://hdl.handle.net/10669/89111
Access Level:acceso abierto
Palabra clave:human antibody
monoclonal antibody
snake venom
neurotoxin
neutralization
VENENOS ANIMALES

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