A Sinorhizobium meliloti and Agrobacterium tumefaciens ExoR ortholog is not crucial for Brucella abortus virulence

 

Guardado en:
Detalles Bibliográficos
Autores: Castillo Zeledon, Amanda, Ruiz-Villalobos, Nazareth, Altamirano-Silva, Pamela, Chacón-Díaz, Carlos, BARQUERO-CALVO, ELIAS, Chaves-Olarte, Esteban, Guzman-Verri, Caterina
Formato: artículo
Fecha de Publicación:2021
Descripción:Brucella is a facultative extracellular-intracellular pathogen that belongs to the Alphaproteo- bacteria class. Precise sensing of environmental changes and a proper response mediated by a gene expression regulatory network are essential for this pathogen to survive. The plant-related Alphaproteobacteria Sinorhizobium meliloti and Agrobacterium tumefaciens also alternate from a free to a host-associated life, where a regulatory invasion switch is needed for this transition. This switch is composed of a two-component regulatory system (TCS) and a global inhibitor, ExoR. In B. abortus, the BvrR/BvrS TCS is essential for intra- cellular survival. However, the presence of a TCS inhibitor, such as ExoR, in Brucella is still unknown. In this work, we identified a genomic sequence similar to S. meliloti exoR in the B. abortus 2308W genome, constructed an exoR mutant strain, and performed its characteri- zation through ex vivo and in vivo assays. Our findings indicate that ExoR is related to the BvrR phosphorylation state, and is related to the expression of known BvrR/BrvS gene tar- gets, such as virB8, vjbR, and omp25 when grown in rich medium or starving conditions. Despite this, the exoR mutant strain showed no significant differences as compared to the wild-type strain, related to resistance to polymyxin B or human non-immune serum, intracel- lular replication, or infectivity in a mice model. ExoR in B. abortus is related to BvrR/BvrS as observed in other Rhizobiales; however, its function seems different from that observed for its orthologs described in A. tumefaciens and S. meliloti.
País:Repositorio UNA
Institución:Universidad Nacional de Costa Rica
Repositorio:Repositorio UNA
Lenguaje:Inglés
OAI Identifier:oai:null:11056/20840
Acceso en línea:http://hdl.handle.net/11056/20840
https://doi. org/10.1371/journal.pone.0254568
Palabra clave:BRUCELOSIS
ZOONOSIS
BACTERIAS
VIRUS
BRUCELLA ABORTUS
VIRUSES