Multidrug-Resistant CTX-M and CMY-2 producing Escherichia coli Isolated from healthy household dogs from the great metropolitan area, Costa Rica
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| Autores: | , , , , , , , |
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| Formato: | artículo |
| Fecha de Publicación: | 2020 |
| Descripción: | Objective:This study aimed to determine the prevalence of fecal carriage of antibiotic-resistantEscherichia coliof healthy household dogs with an emphasis on extended-spectrumb-lactamases (ESBL), AmpC-typeb-lactamases and resistance to quinolones.Materials and Methods:Rectal swabs were collected from 74 dogs without any clinical evidence of gastro-intestinal disease. Samples were cultured on MacConkey agar plates and MacConkey supplemented with2mg/mL cefotaxime or 5mg/mL ciprofloxacin. Isolates were identified with Vitek 2 Compact and susceptibilitytesting performed by Kirby Bauer disk diffusion method. Minimal inhibitory concentration ( MIC) was done onisolates resistant to cefotaxime, ciprofloxacin, and nalidixic acid. PCR amplification was performed to detectCTX-M and CMY-2. Isolates positive for CTX-M and/or CMY-2 were selected for whole-genome sequencing.Results:Multiresistance was detected in 56% of the isolates. A high percentage of resistance was detected forcefazolin (63%), ampicillin (54%), streptomycin (49%), nalidixic acid (42%) and tetracycline (38%). The MIC50andMIC90for isolates resistant to cefotaxime (24%) was determined as 16 and>250mg/mL, respectively; for cipro-floxacin (18%), 125 and 250mg/mL, respectively. ESBL (CTX-M type) and AmpC (CMY-2 type) were detected in 6(7.1%) and 14 (19%) of the isolates, respectively. Whole-genome sequence analysis showed high genetic diversity inmost of the isolates and a large variety of resistance mechanisms, including mobile genetic elements.Conclusion:The frequency of multidrug-resistantE. coliis worrying, mainly because of the presence of manyisolates producing ESBL and AmpCb-lactamases. Based on the ‘‘One Health’’ concept, considering therelationships between animals, humans, and the environment, these data support the notion that companionanimals are important reservoirs of multidrug-resistant bacteria |
| País: | Repositorio UNA |
| Institución: | Universidad Nacional de Costa Rica |
| Repositorio: | Repositorio UNA |
| Lenguaje: | Inglés |
| OAI Identifier: | oai:null:11056/18570 |
| Acceso en línea: | http://hdl.handle.net/11056/18570 |
| Palabra clave: | DOG PERRO ESCHERICHIA COLI ANTIBIÓTICOS ANTIBIOTICS |